Wolverine is definitely neutered, I can see a Deadpool movie being made in PG13, I've said it before, but the R rated is the best way to go imo, that doesn't mean it's going to be a Serbian Film of comicbook movies, and frankly, Deadpool it's most definitely not a family friendly character, he's a mercenary, he kills good people and bad people for money, sure he's somewhat innocent but c'mon.
Besides, it's just not the same standard when it comes to violence between movies and comics.
PS: Just saw the video, I agree with what he said regarding Deadpool, not regarding the rating, Nicieza pretty much nailed it in his tweet, Rambo R + Buggs PG = Deadpool PG 13, that's how it is in the comics, except that's not how it works in movies, you can have Buggs PG comedy, but as soon as you add Rambo's violence, it gets R-rated, it doesn't go in between.
specially in today's political correctness world, you cant have little kids wanting to be deadpool after they see the movie